Yes you can, in the cloning software go to "Continuous Tone" and expand that section. Select "Tone Burst" and select "180 Deg"; you can also customize the timing as well in that section. Also, it's idea to make sure you also have the latest firmware/DSP installed as well as there have been countless fixes added over the years. The latest versions are as follows: CS-FR5000 cloning software is revision 1.9, main firmware is revision 3.2 and DSP is revision 2.9 as of this posting. Good luck and enjoy, there are awesome units and very versatile repeater/base stations.