Since your post says "two way radio" and not a scanner, I'm assuming this radio can transmit and isn't just a receiver?
If this is for a radio that will transmit, then no, you'll need to get an antenna that's resonant on whatever frequency you're using. If you can provide more info as to the make and model of the radio and what frequency you're using, we can provide more information on suggested antennas.
If this is for receive only, then you can use it but it may or may not work very well. Depends on what you're trying to pick up. If the radio is receive only, give it a try and see how well it receives. However, even for a receive-only antenna, it's still best to have an antenna that's resonant on the frequency you're listening on.