If it's only going to lower the amp output, I may not re-tune it right away. I might just run 50 watts into it as long as that won't damage it. I have a 5/8 wave 3dbi gain NMO VHF antenna on the roof of my truck already, so I might even use it with my base setup. Will this possibly change the receiver sensitivity of my radio? I do already have a diplexer in my truck for the separate VHF and UHF antennas, so will combining a diplexer and an amplifier guarantee lowering RX?
It will increase the amp output by retuning it, not lower it. DO NOT RUN 50W into it though!! These are a two stage amp, a driver stage and the final stage. You will blow up the driver stage running 50w into it, even running the max input of 40w makes them run hotter than you know what, i wouldn't recommend more than 20w.
You have two choices.... You can program your VHF memories in the 7800 with the 20w power level selected each time you write the memory so your always running 20w with the amp inline, this is how i setup my amp at home with my FT-8800, or you can modify the amp and bypass the driver stage which i have done as well. Of course that takes a lot of work and technical know-how as well.
It should not do much to the receive sensitivity of the radio, other than of course the usual loss you get from adding additional connectors and such inline. The T/R relay in these do add a bit of loss but its small. You won't lower the receive anymore than you already have using the diplexer, the biggest loss you have in the system is the diplexer, adding the amp will be only a small fraction of the loss.
I run a FT-8800 at home with a pair of amps, a RF Concepts 170w VHF and 100w UHF, i use a diplexer before the amps to split the radio output, and a second diplexer after the amps to combine their output to a single feedline because i run tower mounted antenna switching for selecting various verticals and yagi's. The system works great, i lose a small amount of output power to the diplexer, and a small amount of receive signal, but both amps have a builtin preamp which i adjusted the gain of to compensate for system loss without adding much to the noise floor.
I have other brick amps, both commercial and homebrew for SSB on 2m/70cm as well. I have been tinkering on amps for many years.
Using the diplexer and dual antennas is actually best in your situation. You wouldn't be able to transmit UHF through that amp, you would lose most of the transmitted signal to the filtering and the relay in it, which is very lossy at UHF. And receive would be greatly affected as well. If you ever wanted to use a dual band antenna you could put a diplexer before and after the amp and jumper the UHF ports of the diplexers.