Dan,
I have not heard them for years. I used to hear them on a repeater in the 44-45 range and that has been dead. Opening day used to be very active with both Fish and Game commission running patrols. I have not heard anything for a few years. The actual amount of Fish Commission officers that I have seen has also decreased dramatically the past few years as well. In fact, I have not even seen one officer the past few years on opening day. I am not fishing some remote area either.
I did see a Fish Commission truck, last year. No antennas. I spoke to the officers for a while, as they were setting up nets for a fish survey, and saw no radios.
When I am up in Erie fishing I have seen Fish Commission cars. They have what looks to be low band or high band and 800mhz antennas on the cars. The officers have VHF high band portables. Thanks to my fishing partner exiting Walnut the wrong way, I can confirm without a doubt that they use Erie Sheriff channel 158.790. I got close call hits on the input, near the Fish Commission office at Walnut as well.
Due to their absolute absence on any local freqs, I would guess they have, and it looks like they will also use local systems as well.
From what I have been told, it is a mixed bag as far as the park rangers go as well. Some look to have 800mhz, some sighted with Open Sky radios, some have been heard on local 800mhz systems, and some look to have only VHF.