In most cases the dual DB408's are for dual transmit, with at least one isolator per transmitter per antenna. If a DB420 were used with a hybrid coupler, the same ERP would occur.
When using one for transmit and one for receive, the 30 dB isolation is usually not enough to reduce the transmitter noise and desense to work effective, without adding notch cavities, that add additional losses. Actually, 30 dB isolation is provided by just 3 ft between the nearest dipoles on UHF. With 10 ft between the nearest dipoles the isolation would be about 50 db, and 20 ft would yield 60 dB. The rule of thumb from the old school is to separate the UHF Tx and Rx by at least 15 ft vertical, and use a good receiver preselector to avoid problems under almost any condition.
This is an engineering question. Therefore, it depends upon power out, frequency separation [3 or 5 MHz], actual receiver isolation requirements, and transmitter noise specifications. In addition, you state it is a "multiple repeater trunked" system, so the specs of the proposed receiver and transmitter multicouplers are also important. In short, it may work with just 30 dB isolation, but it is just as likely it may not.
To find out, build the system in the shop and test it local. Be sure to measure the desense using the Motorola method. There is lots of information available on the internet regarding the amount of isolation required for proper operation.