I don't recall ever hearing them on the repeater. I think they can only use 145.8 but I'm not sure. The AMSAT page doesn't specify.
In the past, ISS crews would talk on 145.800 MHz, and listen to ground stations on either 145.200 MHz when over ITU region 1 (Europe, Africa, & ex-USSR countries), or 144.490 MHz when over the rest of the world. NASA astronaut Kjell Lindgren KO5MOS operated as NA1SS last year on the ISS cross-band repeater. It's easy for them to join in when the repeater is active - turn up the volume on the TM-D710G, pick up the microphone, and join in. And it helps ground stations, as the repeater allows everyone to hear all of the activity, and not just the ISS side of contacts.
If you want to hear some of the NA1SS activity on the ISS cross-band repeater from last year, look at some of the videos on my YouTube channel
http://www.youtube.com/va7ewk titled "NA1SS on the ISS cross-band repeater". An example of an ISS pass with NA1SS on the repeater from last June is at:
IIRC the new Kenwood radios on the ISS - one in the Russian service module (normally runs packet on 145.825 MHz, and previously SSTV on 145.800 MHz), and the other in the European Columbus lab (normally running the cross-band repeater - uplink on 145.990 MHz with 67.0 Hz tone, downlink on 437.800 MHz +/- for Doppler) - still support the 2m split-frequency pairs previously used by crews. Hopefully we will hear crews picking up the mic and joining in on the repeater.
73!