AFAIK, I believe this can be done with something like QEMU. The main issue is that RPi has an ARM processor, and most PCs use x86-64 architecture, so that's the big problem. QEMU can emulate an ARM setup, but I don't know what the performance would be like realistically. I've seen a few tutorials on doing this, but it was written while using Ubuntu as the host operating system, so I am unsure if doing this in Windows if feasible or not.
In this article we see how to run the official Raspberry Pi Os in a virtual machine using qemu and Kvm
linuxconfig.org
What might be much simpler, and help keep your sanity intact, would be to make an x86 Linux VM (Debian, LM20, Ubuntu 20.04) and just install the software on that first and copy over your relevant config files (like OP25 json, tsv, and startup scripts) from your Raspberry Pi to the new setup.