It's a check (Group Affiliation Query, technically) to see if a subscriber is still affiliated with X talkgroup at Y site. Motorola ASTRO 25 systems send the queries out 12* hours after the subscriber's last activity, and they will only be sent out if the subscriber did not de-register. The "Ack" message you see in Unitrunker is the subscriber acknowledging the query.
So in plain English, the system is checking to see if the radio is still out there. Every time there is some activity from the radio (keying up, data calls, changing talkgroups, roaming between sites) that clock is reset. If the radio properly de-registers, the clock stops. That doesn't always happen, though, and so a radio may go out of range then be powered down so it wouldn't have de-registered. Or a battery is pulled off a portable before powering it down. Or a mobile is installed in such a way that turning off the ignition switch abruptly cuts power to the radio. Etc.
* 12 hours is the default duration, but that can be changed if desired