Assuming you're talking about a x500 with remote head; the installation manual states that a mic should not be plugged into the programming port. My guess is that Motorola uses pins on that port for interesting functions they don't want to have available on the front panel mic port.
I've never seen anything in CPS that would indicate you can use the programming port on the chassis for a mic.
However, if you mean the oversized DB26 accessory port on the back (which you can also program through), you could hook a mike up to that, but you would have to wire it up yourself. I gave the pinout in another thread.
I need some help with pinout and programming on an APX6500 O5 Dash Mount. I need to interface the radio to a Harris UAC Interoperability Gateway with Audio In, Audio Out, PTT and COR. Does anyone have the pinout for the rear connector and Digital In/Out programming area to match the PTT/COR...
forums.radioreference.com
You'll probably want Pins 14, 16 and 23, possibly others.