Can you open a shell and report back the results for the two commands:
arecord -l
aplay -l
Paste the output and let me know.
Here ya go. Sorry I had to step out for a bit ..
**** List of CAPTURE Hardware Devices ****
card 0: D1 [Loopback], device 0: Loopback PCM [Loopback PCM]
Subdevices: 1/1
Subdevice #0: subdevice #0
card 0: D1 [Loopback], device 1: Loopback PCM [Loopback PCM]
Subdevices: 1/1
Subdevice #0: subdevice #0
card 2: Device [USB Audio Device], device 0: USB Audio [USB Audio]
Subdevices: 0/1
Subdevice #0: subdevice #0
**** List of PLAYBACK Hardware Devices ****
card 0: D1 [Loopback], device 0: Loopback PCM [Loopback PCM]
Subdevices: 1/1
Subdevice #0: subdevice #0
card 0: D1 [Loopback], device 1: Loopback PCM [Loopback PCM]
Subdevices: 1/1
Subdevice #0: subdevice #0
card 1: Headphones [bcm2835 Headphones], device 0: bcm2835 Headphones [bcm2835 Headphones]
Subdevices: 8/8
Subdevice #0: subdevice #0
Subdevice #1: subdevice #1
Subdevice #2: subdevice #2
Subdevice #3: subdevice #3
Subdevice #4: subdevice #4
Subdevice #5: subdevice #5
Subdevice #6: subdevice #6
Subdevice #7: subdevice #7
card 2: Device [USB Audio Device], device 0: USB Audio [USB Audio]
Subdevices: 1/1
Subdevice #0: subdevice #0
I also just added the second sound card and my 2nd instance started right up. I tried to reconfigure the 1st and here is what I got
>>> Enabling darkice_1 to start on boot
Done. Press [Enter] to continue...
Configuration Finished!
Do you want to start this darkice broadcast now? [N/y]: y
Starting darkice_1...
● darkice_1.service - Darkice Instance 1 Service
Loaded: loaded (/etc/systemd/system/darkice_1.service; enabled; vendor preset: enabled)
Active: activating (auto-restart) (Result: exit-code) since Tue 2021-04-20 16:43:54 CDT; 1s ago
Process: 1083 ExecStartPre=/bin/sleep 10 (code=exited, status=0/SUCCESS)
Process: 1084 ExecStart=/usr/bin/sh -c /usr/local/bin/darkice -v5 -c /home/pi/bcfy/configs/darkice/darkice_1.cfg > /home/pi/bcfy/logs/darkice_1.log 2>&1 (code=exited, status=255/EXCEPTION)
Main PID: 1084 (code=exited, status=255/EXCEPTION)
Press [Enter] to return to the main menu...